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NovAbstract:
All organisms, including humans, possess a huge number of uncharacterized enzymes. Here we describe a general cell-based screen for enzyme substrate discovery by untargeted metabolomics and its application to identify the protein alpha/beta-hydrolase domain-containing 3 (ABHD3) as a lipase that selectively cleaves medium-chain and oxidatively truncated phospholipids. Abhd3(-/-) mice possess elevated myristoyl (C14)-phospholipids, including the bioactive lipid C14-lysophosphatidylcholine, confirming the physiological relevance of our substrate assignments.
Notes:
Long, Jonathan ZCisar, Justin SMilliken, DavidNiessen, SherryWang, ChuTrauger, Sunia ASiuzdak, GaryCravatt, Benjamin FCA132630/CA/NCI NIH HHS/R01 CA132630-04/CA/NCI NIH HHS/Nat Chem Biol. 2011 Sep 18;7(11):763-5. doi: 10.1038/nchembio.659.