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Phosphonium labeling for increasing metabolomic coverage of neutral lipids using electrospray ionization mass spectrometry

Citation:

Woo HK, Go EP, Hoang L, Trauger SA, Bowen B, Siuzdak G, Northen TR. Phosphonium labeling for increasing metabolomic coverage of neutral lipids using electrospray ionization mass spectrometry. Rapid Commun Mass Spectrom. 2009;23 :1849-55.

Date Published:

Jun

Abstract:

Mass spectrometry has become an indispensable tool for the global study of metabolites (metabolomics), primarily using electrospray ionization mass spectrometry (ESI-MS). However, many important classes of molecules such as neutral lipids do not ionize well by ESI and go undetected. Chemical derivatization of metabolites can enhance ionization for increased sensitivity and metabolomic coverage. Here we describe the use of tris(2,4,6,-trimethoxyphenyl)phosphonium acetic acid (TMPP-AA) to improve liquid chromatography (LC)/ESI-MS detection of hydroxylated metabolites (i.e. lipids) from serum extracts. Cholesterol which is not normally detected from serum using ESI is observed with attomole sensitivity. This approach was applied to identify four endogenous lipids (hexadecanoyl-sn-glycerol, dihydrotachysterol, octadecanol, and alpha-tocopherol) from human serum. Overall, this approach extends the types of metabolites which can be detected using standard ESI-MS instrumentation and demonstrates the potential for targeted metabolomics analysis.

Notes:

Woo, Hin-KoonGo, Eden PHoang, LinhTrauger, Sunia ABowen, BenjaminSiuzdak, GaryNorthen, Trent RMH062261/MH/NIMH NIH HHS/P30 MH062261-10/MH/NIMH NIH HHS/EnglandRapid Commun Mass Spectrom. 2009 Jun;23(12):1849-55.

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