Agilent 6210 Time-of-Flight LC/MS This is primarily a high resolution mass spectrometer (~6,000 at m/z 622) which is used for both nominal and exact mass measurement (< 5 ppm). It uses an internal lock-mass correction to provide very high accuracy measurments. It's linear dynamic range is between 2 to 3 orders of magnitude. An Agilent 1100 series HPLC with a binary pump and autosampler are coupled to this mass spectrometer for performing both rapid flow injection analysis (FIA) and LC-MS experiments. This instrument is available on an open access basis. Agilent EasyAccess software provides an easy user interface for open access use.
Waters Quattro micro GC/MS/MS Is a triple quadrupole mass spectrometer with an Agilent 6890 GC and CTC CombiPAL autosampler has a mass range from 4 to 1500 amu. Both 70 eV electron ionization (EI) and chemical ionization (CI) with methane or ammonia as reagent gas. As a triple quad mass spectrometer, it can be used for many methods such as multiple reaction monitoring (MRM), pproduct ion, precursor ion and neutral loss/gain. However, many users prefer to do simple GC-MS experiments for volatile analytes (typically <500 amu in MW). As a "hard" ionization technique, EI produces abundant fragment ions. This can be highly useful since the fragments produced can be used for structural characterization. Our GC-MS comes equipped with the NIST spectral library that contains hundreds of thousands of spectra and which can be searched for structural identification of unknowns. This mass spectrometer is staff-run only.
The Waters Micro MX MALDI-TOF. This instrument offers both reflectron (isotopic resolution < 4000 Da) and linear mode detection (up to 100 kDa) and can be used for the analysis of peptides, oligonucleotides, proteins and many other small and large molecular weight determinations. Due to the "soft" ionization of the MALDI source and low charge state typically observed, the mass range can vary from below 100Da to well over 250,000Da. The instrument is available on an open access basis once training has been completed, and is a popular instrument for the students due to it's simplicity and reliability.
Agilent 1100 HPLC. An analytical scale HPLC with a fraction collector which can be used to separate and analyze components within a chemical mixture. The injection volume of the autosampler is 1-100 uL. The pump flow rate is 0.1-2 mL/min. Chemstation software is used for control and data analysis. This is available to users on an open access basis.
Bruker Maxis Impact LC-q-TOF Mass Spectrometer. This is a next generation high accuracy (sub-ppm with internal calibration), high resolution (40,000 resolving power) tandem mass spectrometer, and ion funnel optics for maximum sensitivity. It's high scan rate allows excellent the characterization of complex mixtures such as with untargeted metabolomics experiments. It is able to obtain high resolution MS/MS spectra at 50 Hz! It has a dynamic range of 4 orders of magnitude and comes equipped with CaptivesprayTM nanoelectropray, conventional flow ESI and APCI for maximum flexibility. This instrument is staff-run only.
Agilent 6460 Triple Quadrupole Mass Spectrometer with Agilent 1290 uHPLC (Two Systems in-House) We use this to perform highly sensitive quantification and targeted metabolomics experiments using MRM. The new Jetstream electrospray source and an atmospheric pressure chemical ionization (APCI) source allows maximum flexibility in monitoring a range of molecules. It is equipped with an Agilent 1290 uHPLC and is staff run only.
The New ultrafleXtreme MALDI-TOF/TOF Mass Spectrometer
This matrix assisted laser desorption ionization (MALDI-TOF/TOF) mass spectrometer is arguably the best in class on the market. It combines true 2 kHz speed in TOF mode and 1 kHz in TOF/TOF mode with ultrahigh performance and extreme flexibility for a broad variety of complementary research, clinical and applied proteomics applications. With the added capability of fragmentation, you can finally characterize the structure for your MALDI generated ions. Also, good performance in linear mode allows you to analyze large biomolecules. This instrument is open access.
Agilent 6550 q-TOF-NEW high resolution tandem mass spectrometer comes with a Jetstream dual-ESI and ion-funnel ion optics for maximum sensitivity. This is a staff-run only instrument that can be used for untargeted global metabolite profiling, as well as analysis of small molecules, intact proteins or oligonucleotides. It yields high resolution/high accuracy tandem mass spectra (MS/MS).
Thermo q-Exactive Plus with Protein Mode-New This q-Orbitrap (FTMS) mass spectrometer is capable of high resolving power (140,000), and high resolution MS/MS. It is highly sensitive and able to switch quickly between positive and negative ion mode. It comes with the protein mode option to facilitate the analysis of larger biomolecules which need an optimized pressure in the collision cell for maximum sensitivity. We also have Thermo's protein deconvolution software to facilitate the calculation of the neutral molecular weight from multiple charge states. It is coupled to an Ultimate 3000 uHPLC. This instrument is staff-run only.
Nitrogen Evaporator from Oranomation with 24 ports This device is specifically for gently drying down large amounts of solvents for example in a metabolite extraction. It enables the drying down of up to 24 samples at a time gently and in large volumes, while carefully controlling the nitrogen flow using a flow meter, and temperature using a water bath. It can be used following a liquid-liquid extraction where samples need to be dried down before reconstitution in a known volume of solvent. This is open to our users to prepare samples.