Agilent 6210 Time-of-Flight LC/MS This is primarily a high resolution mass spectrometer (~6,000 at m/z 622) which is used for both nominal and exact mass measurement (< 5 ppm). It uses an internal lock-mass correction to provide very high accuracy measurments. It's linear dynamic range is between 2 to 3 orders of magnitude. An Agilent 1100 series HPLC with a binary pump and autosampler are coupled to this mass spectrometer for performing both rapid flow injection analysis (FIA) and LC-MS experiments. This instrument is available on an open access basis. Agilent EasyAccess software provides an easy user interface for open access use.
Waters Quattro micro GC/MS/MS Is a triple quadrupole mass spectrometer with an Agilent 6890 GC and CTC CombiPAL autosampler has a mass range from 4 to 1500 amu. Both 70 eV electron ionization (EI) and chemical ionization (CI) with methane or ammonia as reagent gas. As a triple quad mass spectrometer, it can be used for many methods such as multiple reaction monitoring (MRM), pproduct ion, precursor ion and neutral loss/gain. However, many users prefer to do simple GC-MS experiments for volatile analytes (typically <500 amu in MW). As a "hard" ionization technique, EI produces abundant fragment ions. This can be highly useful since the fragments produced can be used for structural characterization. Our GC-MS comes equipped with the NIST spectral library that contains hundreds of thousands of spectra and which can be searched for structural identification of unknowns. This mass spectrometer is for fee-for-service work only.
Thermo Scientific* TSQ Quantum Access MAX triple stage quadrupole mass spectrometer. This mass spectrometer offers a large linear dynamic range of 6 orders of magnitude and a m/z range of up to 1500. It is ideal for selective quantitation experiments with techniques such as multiple reaction monitoring (MRM). We use this mass spectrometer for targeted metabolomics and other quantification experiments on a fee-for-service basis. This instrument is not available to open access users.
The Waters Micro MX MALDI-TOF. This instrument offers both reflectron (isotopic resolution < 4000 Da) and linear mode detection (up to 100 kDa) and can be used for the analysis of peptides, oligonucleotides, proteins and many other small and large molecular weight determinations. Due to the "soft" ionization of the MALDI source and low charge state typically observed, the mass range can vary from below 100Da to well over 250,000Da. The instrument is available on an open access basis once training has been completed, and is a popular instrument for the students due to it's simplicity and reliability.
Agilent 1100 HPLC. An analytical scale HPLC with a fraction collector which can be used to separate and analyze components within a chemical mixture. The injection volume of the autosampler is 1-100 uL. The pump flow rate is 0.1-2 mL/min. Chemstation software is used for control and data analysis. This is available to users on an open access basis.
Agilent 6120 Quadrupole LC/MS with Multimode source A single quadrupole instrument capable of unit mass resolution to 1500 amu. The instrument is equipped with a Multimode source capable of simultaneous APCI and ESI ionization in both positive and negative ion modes. Samples that ionize in solution are generally analyzed in ESI-only mode; those that do not are generally analyzed in APCI-only mode. Samples with poorly defined or unknown characteristics can be analyzed in Multimode combining ESI and APCI in a single run. The instrument is configured for either flow injection or HPLC introduction of samples from an automatic sampler. The mass spectrometer is equipped with a fraction collection system allowing peak collection based on a specific mass, total ion current, UV absorbance, or a combination the three. Preparative chromatography can be accommodated at flow rates up to 5 mL/min. This mass spectrometer has a large linear dynamic range of approximately 6 orders of magnitude and is highly suitable for quantitation studies. This is not an open access mass spectrometer but we can design and run specific experiments for you on this instrument on a fee-for-service basis.
Bruker Maxis Impact LC-q-TOF Mass Spectrometer. This is a next generation high accuracy (sub-ppm with internal calibration), high resolution (40,000 resolving power) tandem mass spectrometer. It's high scan rate allows excellent the characterization of complex mixtures such as with untargeted metabolomics experiments. It is able to obtain high resolution MS/MS spectra at 50 Hz! It has a dynamic range of 4 orders of magnitude and comes equipped with CaptivesprayTM nanoelectropray, conventional flow ESI and APCI for maximum flexibility. This instrument will be used for special projects requiring high mass accuracy and resolution on a fee-for-service and collaborative basis.
Agilent 6460 Triple Quadrupole Mass Spectrometer with Agilent 1290 uHPLC
This LC-MS/MS instrument is expected to boost our capacity for sensitive and accurate quantitation of molecules using multiple reaction monitoring (MRM) and other mass spectrometry techniques that allow the highest dynamic range (5 to 6 orders of magnitude) and most sensitive measurements possible at an expanded MW range of 50-3200 m/z. Other unique experiments which can be performed on this high performing triple quad mass spectrometer include precursor ion scanning, neutral loss scans etc. which can target a specific class of compounds in a sample depending on its tandem MS pattern. It comes with an Agilent 1290 uHPLC which can be used for rapid and sensitive HPLC methods development using ultra high performance (and pressure) HPLC. The new Jetstream electrospray source and an atmospheric pressure chemical ionization (APCI) source will allow maximum flexibility in monitoring a range of molecules. This instrument is expected to be a crucial tool for targeted metabolomics research. This instrument will be operated on a fee-for-service and collaborative basis.
The New ultrafleXtreme MALDI-TOF/TOF Mass Spectrometer
This matrix assisted laser desorption ionization (MALDI-TOF/TOF) mass spectrometer is arguably the best in class on the market. It combines true 2 kHz speed in TOF mode and 1 kHz in TOF/TOF mode with ultrahigh performance and extreme flexibility for a broad variety of complementary research, clinical and applied proteomics applications. With the added capability of fragmentation, you can finally characterize the structure for your MALDI generated ions. Also, good performance in linear mode allows you to analyze large biomolecules.