Please make sure that your samples are completely in-solution before analysis on ESI-TOF or any LC-MS instrument. Centrifuge at 14000 g to remove any possible solids. Any particulate matter will clog the small inner diameter transfer lines, injector etc. and cause maintenance down time. Spinning using a high speed ultracentrifuge and transferring the supernatant should be used to remove solids.
We now have an in-house XCMS server. XCMS is an integrated metabolomics platform that facilitates the comparative analysis of LC-MS profiles identify significant differences between two or more sets of samples. It is also integrated with Metlin metabolite database to help detect candidate metabolites through their accurate mass measurements. Replicates can be measured for each set of samples, for example control vs.
There has been some confusion about which calendar to check! Please check the hourly reservation calendar, not the slot based reservations. The slot based reservations are really not time linked so the only calendar that is important is the hourly calendar for the Agilent ESI-TOF. You don't even need to login, just go to the the following link! Before walking over to use the "walk-up" instruments, just check the