Our Northwest Laboratories Building Operations has just installed a new Parker Hannifin Nitrogen Generator system that is fully redundant and can supply >250 L/minute of ultra-high purity nitrogen for our mass spectrometers. This is an impressive system that was designed by Jim Sheehan and his staff and is going to make our lab even more efficient. We used to rely on boil-off from liquid nitrogen dewars which does not have a consistent output, and causes a lot of wasted cryogen. Read more about Parker Hannifin Nitrogen Generator System
Our Waters MALDI-TOF lost its Penning gauge (device for measuring pressure) when coming back up from a system vent. We were able to replace it this week and it is now pumped down and ready to use. If you would like to use it, please proceed through the normal SPINAL reservation and try it out. Let me, Sunia know if you experience any problems. Also, if you notice it is not working, please let us know so we can try to fix it.
In response to popular demand, bulk rates will be offered to non-profit organizations when a large number of LC-MS or GC-MS samples are submitted for running with the same method. This type of discount was already offered to Harvard University researchers, and will now be extended to non-profit organizations. Please note, the non-profit rates are always higher to recover the overhead rate paid on government grants which partially subsidize our core facility. For details on what you will pay as a researcher from a non-Harvard non-profit organization, please check out the following link: Read more about Multiple Sample Discounts for Non-Profits
We are grateful to Thermo for placing a "seed" q-Exactive in our lab for metabolomics applications. This is a high resolution FT mass spectrometer based on Orbitrap technology which is capable of obtaining ~140,000 resolution. The instrument will come with a Thermo uHPLC for LC-MS experiments. We look forward to the opportunity of investigating how the unique features of this instrument can benefit and complement existing technologies in our lab.
We just purchased a 24 port nitrogen evaporator from Organomation. This allows us to gently and simultaneously dry down and concentrate metabolite extracts prior to mass spectrometry analysis. In contrast to a speedvac, you can use larger glass vials/tubes, and can control the temperature with a water bath.
The Bruker Impact q-TOF will undergo a significant hardware upgrade and become a Impact HD on Monday February 3 and February 4 . This upgrade involves the installation of a more powerful turbo pump for better vacuum, and the change of the digitizer from a 8 bit to a 10 bit board. What this means for us is that we will now have significantly higher dynamic range, and better sensitivity for proteins. The detector will not saturate as easily with concentrated samples, letting us see the low level components in a complex mixture. Read more about Upgrade for the Bruker q-TOF
If you have data from our Bruker q-TOF Impact and want to view the data, ask Sunia about the DA Viewer license free version. She can help you set it up on your computer. Also, DA Viewer has now been placed on the computer adjacent to the Agilent ESI-TOF for data viewing so you can use it to view your Bruker data.
Due to the heavy demand for the ESI-TOF for high resolution work, we have decided to leave a two hour block open daily between 10 AM to 12 PM. During this time, you can run quick flow injection analysis (direct injection/no LC) experiments by using the Easy Access software. This ensures a time every day where people can do simple high resolution work. Easy Access queues up samples so it is first-come-first serve!
Due to heavy use of the Agilent ESI-TOF, some people were not able to run their quick direct injection experiments on certain days. While many of you would probably like a dedicated "open access" instrument, this is not possible or an efficient use of the instrument, since on some days, no one runs their samples. Our alternate solution, I believe will assure a more efficient use of the instrument, while still allowing people to run their quick samples daily. Read more about NEW Open Access Hours 10-12pm for Quick Experiments